The Annandale Distillery was closed by owners Johnnie Walker in 1919 but after a multi-million pound investment whisky is being produced once more at the venue.
Owners praised the malt whisky of the 'finest quality and character' after drawing the whisky three years to the day from filling their first barrel.
Managing director Professor David Thomson performed the ceremony, which was streamed lived on the distillery's Facebook.
Bottles of the peated 'Man o' Swords' whisky were drawn off from the first cask at the site, which is near the town of Annan.
The product, which is named in honour of Robert the Bruce, will join the unpeated 'Man o' Words' whisky, which is a tribute to Robert Burns.
Prof Thomson told the BBC: "Whisky distillation isn't new to Dumfriesshire.
"In the latter part of the 19th Century, three distilleries were producing locally; taking advantage perhaps of the abundance of peat for malting barley.
"Alas, all had closed by 1920."
Teresa Church, who co-owns the distillery, added: "Present day Annandale Distillery exists in a different world from that of once familiar horse carts and water wheels and therefore has to step into a global technological arena to be successful.
"The proud provenance and brand story of The Man o' Words and Man o' Sword will hopefully build strong brand equity in the modern-day global market place."