The Subway stores which are reopening - which total about a quarter of all UK Subway outlets - will offer delivery through Just Eat, Uber Eats and Deliveroo.
The stores closed in March amid the UK lockdown restrictions.
Safety measures will be in place, including enhanced hygiene practises, PPE equipment to ensure social distancing and directional signage.
The brand says that these procedures will ensure the branches, which operate on a franchise model, are able to trade responsibly.
The measures have already been tested in the small number of stores that have remained open to support and serve key workers and hospital staff over recent weeks.
Colin Hughes, country director for Subway UK & Ireland said: “Everybody in our Subway family and the wider communities they serve, has shown great resilience in adapting to these challenging times that we currently find ourselves in.
“We are extremely grateful for their ongoing support in anticipation of this next phase of our gradual reopening, which will enable us to continue to serve our communities and frontline workers, who have helped us all so much in recent weeks.
“We have slowly and safely introduced a new store operating framework that will be rolling out across stores to protect guests, Sandwich Artists, delivery drivers and our suppliers.
"The weeks and months ahead will continue to be challenging for everyone and Subway will play its part in supporting the new normal whatever that may look and feel like.”
Subway currently offers a 25 per cent discount all to NHS staff and key workers and has donated over 250,000 sandwiches to those most in need.
Customers who want to find out if their local Subway store is reopening can visit the brand’s website.
Subway is also currently offering free delivery on all third party orders over £10 – this includes Just Eat, Uber Eats and Deliveroo – and customers can place orders via the brand’s Subcard Loyalty App to then collect in store.