The Macallan Royal Marriage Malt Whisky was blended from two single malts distilled in 1948 and 1961 - the birth years of Charles and Diana.
It was bottled in Scotland 41 years ago and released to celebrate the Royal Wedding on July 29, 1981.
The label shows two whisky casks nestled side by side bearing the years 1948 and 1961 together with the words: 'Long Life and Happiness'.
It reads: "To mark the historic occasion of the wedding of His Royal Highness The Prince of Wales & Lady Diana Spencer, the directors of Macallan-Glenlivet Limited respectfully present this loyal celebration vatting: a unique marriage of our fine single malt whisky distilled in 1948 and 1961, respectively the years of the Royal couple's births."
The limited-edition bottle is now going under the hammer at Hansons Auctioneers and is expected to fetch between £1,800 and £2,000.
Auctioneer Charles Hanson said: "Macallan is world-renowned for its fine whisky and this rare limited-edition Royal Marriage Malt is likely to be sought after.
"This whisky is a reminder of a joyful moment.
"It's a rare bottle to find and we've seen it offered by whisky retailers for prices approaching £10,000.
"For any whisky connoisseur or collector, it's an impressive addition to the drinks cellar and an investment item."
Charles and Diana separated in 1992 and divorced in 1996. The following year Diana was tragically killed in a car accident in Paris.
The 75cl bottle of whisky will be auctioned on Friday (19/8).
